Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US4808532

Title:Continuous human cell lines and method of making same
Inventors:Stampfer, Martha R.; Oakland, CA, USA
Summary:This invention describes continuous human cell lines which are derived from normal human mammary epithelial cells (HMECs) according to a method of treatment with a chemical carcinogen. Described herein are genetically stable continuous human cell lines derived from normal HMECs and processes for their production and use. Further disclosed are methods by which cell lines are derived from such HMECs by treating normal human mammary epithelial tissue with a chemical carcinogen that is a polycyclic aromatic hydrocarbon such as benzo[a]pyrene. Multiple embodiments of the invention are included. Also provided are techniques by which the novel cell lines may serve as useful substrates for elucidating the potential effects of a number of toxins, carcinogens and mutagens and of additional exogenous genetic material. Methods are further included by which the autogenic parent cells from which the cell lines are derived may serve as the control samples for testing.
Abstract:
US Patent Website:Click Here for Full Text of Patent
Title Number:US4808532
Application Number:US1985000750124
Date Filed:01/07/1985
Date Published:28/02/1989
Assignee:The USA as represented by the United States Department of Energy, Washington, DC, USA


 
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