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Stem Cell Related Patent Number US4959313

Title:Cellular enhancer for expressing genes in undifferentiated stem cells
Inventors:Taketo, Makoto; Bar Harbor, ME, USA
Summary:Described herein is a novel cellular enhancer nucleotide sequence and methods of its use in the expression of genes in undifferentiated stem cells. The invention relates to a cellular enhancer isolate which has been shown to cause expression in undifferentiated stem cells of a flanking exogenous or recombinant gene from a promoter having a specific phenotypic trait and which accompanies the gene wherein the gene and promoter are not normally expressed in undifferentiated stem cells. Further disclosed are recombinant vectors including plasmids and viruses and methods by which they may be constructed in such a way that they contain the novel cellular enhancer. Also provided are mechanisms by which propagating cells containing the vector constructs have been shown to reproduce and propagate the vectors.
Abstract:A novel cellular enhancer nucleotide sequence causes expression in undifferentiated stem cells of a flanking exogenous or recombinant gene from a promoter accompanying the gene where the gene and promoter are not normally expressed in the undifferentiated stem cells. In the preferred example the essential or basic core of the cellular enhancer nucleotide sequence is ##STR1## The cellular enhancer may encompass a more general core sequence of approximately, for example, in the range of 300 to 350 bases including the essential or basic core sequence. Recombinant vectors including plasmids and viruses are constructed bearing the novel cellular enhancer flanking a recombinant or exogenous gene and promoter having a specified phenotypic trait to be expressed in undifferentiated stem cells. Propagating cells containing the vector constructs reproduce and propagate the vectors. Undifferentiated stem cells including cell lines and animals express the desired phenotypic trait of an exogenous or recombinant gene under the control of the novel cellular enhancer.
US Patent Website:Click Here for Full Text of Patent
Title Number:US4959313
Application Number:US1987000064665
Date Filed:22/06/1987
Date Published:25/09/1990
Assignee:The Jackson Laboratory, Bar Harbor, ME, USA


 
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