Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US5162215

Title:Method of gene transfer into chickens and other avian species
Inventors:Bosselman, Robert A.; Thousand Oaks, CA, USA
Hu, Shaw-Fen S.; Newbury Park, CA, USA
Nicolson, Margery A.; Pacific Palisades, CA, USA
Summary:Herein are described methods for the transfer of genes into avian species according to a process by which a replication-defective retroviral vector is utilized in embryonic pluripotent stem cells. The invention relates to a process by which the replication-defective retroviral vector is introduced into pluripotent stem cells of avian embryos in the absence of an exogenous replication-competent helper retrovirus, resulting in the transfer of nucleic acid sequences which may differentiate into somatic or germ cells. Further disclosed are methods by which transgenic nonhuman mammals may be produced from the germ cells. Therapeutic and experimental applications are included.
Abstract:A method for introducing a replication-defective retroviral vector into pluripotent stem cells of embryos of an avian species, including chickens, turkeys, quails or ducks. The method is useful for transferring nucleic acid sequences into embryonic avian cells which may differentiate into somatic or germ cells. Transfer into germ cells has been achieved to produce transgenic animals. The replication-defective retroviral vector used for transfer may be a recombinant retroviral vector containing both a retroviral derived nucleic acid sequence and a non-retroviral derived nucleic acid sequence. Examples of non-retroviral nucleic acid sequences are a neomycin resistance gene from the bacterial transposon Tn5, a herpes simplex virus thymidine kinase gene and a chicken growth hormone gene, however, any prokaryotic or eukaryotic nucleic acid sequence of interest may be used. Transgenic chickens have been produced whose cells contain and express a replication-defective retroviral vector nucleic acid sequence.
US Patent Website:Click Here for Full Text of Patent
Title Number:US5162215
Application Number:US1988000247964
Date Filed:22/09/1988
Date Published:10/11/1992
Assignee:Amgen Inc., Thousand Oaks, CA, USA Arbor Acres Farm, Inc., Glastonbury, CT, USA


 
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