Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US5199942

Title:Method for improving autologous transplantation
Inventors:Gillis, Steven; Mercer Island, WA, USA
Summary:Described herein are methods of using hematopoietic progenitor cells for autologous cell transplantation. The invention relates to methods by which such progenitor cells are expanded exo vivo in the presence of a growth factor for hematopoietic rescue after cytoreductive therapy. Further disclosed are methods by which hematopoietic progenitor cells are obtained from bone marrow or peripheral blood from a patient prior to cytoreductive therapy, after which the hematopoietic progenitor cells are expanded ex vivo with a growth factor selected from the group consisting of interleukin-3 (IL-3), steel factor (SF), granulocyte macrophage-colony stimulating factor (GM-CSF), interleukin-1 (IL-1), GM-CSF/IL-3 fusion proteins or any combination thereof, from which a cellular preparation is obtained that is composed of an expanded population of progenitor cells which is then administered to the patient concurrently with or following cytoreductive therapy.
Abstract:There is disclosed a method for autologous hematopoietic cell transplantation of patients receiving cytoreductive therapy, comprising: (1) obtaining hematopoietic progenitor cells from bone marrow or peripheral blood from a patient prior to cytoreductive therapy; (2) expanding the hematopoietic progenitor cells ex vivo with an ex vivo growth factor selected from the group consisting of interleukin-3 (IL-3), steel factor (SF), granulocyte macrophage-colony stimulating factor (GM-CSF), interleukin-1 (IL-1), GM-CSF/IL-3 fusion proteins and combinations thereof, to provide a cellular preparation comprising an expanded population of progenitor cells; and (3) administering the cellular preparation to the patient concurrently with or follwoing cytoreductive therapy. The inventive method optionally comprising a preliminary treatment with a recruitment growth factor to recruit hematopoietic progenitor cells into peripheral blood and a subsequent treatment with an engraftment growth factor to facilitate engraftment and proliferation of hematopoietic progenitor cells administered in the cellular preparation. The invention further provides a hematopoietic progenitor cell expansion media composition comprising cell media, an ex vivo growth factor, and autologous serum.
US Patent Website:Click Here for Full Text of Patent
Title Number:US5199942
Application Number:US1991000765844
Date Filed:26/09/1991
Date Published:06/04/1993
Assignee:Immunex Corporation, Seattle, WA, USA


 
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