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Stem Cell Related Patent Number US5225353
| Title: | Method for immunoselection of cells using avidin and biotin | | Inventors: | Berenson, Ronald J.; Bellevue, WA, USA
Bensinger, William I.; Seattle, WA, USA | | Summary: | Described herein are methods of immunoselection that are specific to cell populations and in which a biotin-conjugated anti-species immunoglobulin and insolubilized avidin is employed. The invention relates to the selectivity of immunoselection systems that utilize insolubilized avidin and in which a biotinylated specific antibody is amplified. Claims of the invention include improved nonspecific recovery by employing an indirect sandwich technique using a biotin-conjugated antispecies immunoglobulin that is directed to one or more nonbiotinylated specific antibodies in conjunction with insolubilized avidin. Further disclosed are methods by which specific cell populations may be removed and recovered from bone marrow thereby yielding recovery of bone marrow and preservation of hematopoietic stem cells for therapeutic transplantation. This invention represents a continuation of other applications filed by the same assignee and inventors. | | Abstract: | The selectivity of immunoselection systems employing insolubilized avidin and biotinylated specific antibody is amplified, and nonspecific recovery is improved, by employing an indirect sandwich technique using a biotin-conjugated antispecies immunoglobulin that is directed to one or more nonbiotinylated specific antibodies in conjunction with insolubilized avidin. Specific cell populations can be removed and recovered from bone marrow, providing excellent recovery of bone marrow and preservation of hematopoietic stem cells for transplantation. Mixed populations of T cells or of tumor cells can be conveniently and simultaneously removed with minimal manipulation of the marrow cells. An improved positive immunoselection method provides viable and functional recovered cells, e.g., hematopoietic stem cells or activated killer cells, that can be clinically employed. | | US Patent Website: | Click Here for Full Text of Patent | | Title Number: | US5225353 | | Application Number: | US1991000769530 | | Date Filed: | 03/10/1991 | | Date Published: | 06/07/1993 | | Assignee: | Fred Hutchinson Cancer Research Center, Seattle, WA, USA |
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