Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US5340740

Title:Method of producing an avian embryonic stem cell culture and the avian embryonic stem cell culture produced by the process
Inventors:Petitte, James N.; Raleigh, NC, USA
Yang, Zengming; Raleigh, NC, USA
Summary:Described herein is a method of culturing avian embryonic stem cells, uses and advantages thereof. The invention relates to a process by which avian blastoderm cells are grown on a mouse fibroblast feeder cell layer in a medium containing a leukemia inhibitory factor, such that undifferentiated avian cells expressing an embryonic stem cell phenotype are produced. Further disclosed are methods by which avian cells are collected from an avian blastoderm prior to the formation of the primitive streak, after which the avian cells are deposited in contact with a mouse fibroblast feeder cell layer and grown in the presence of a leukemia inhibitory factor in a differentiation-inhibiting amount for a time sufficient to produce a sustained avian cell culture. Associated veterinary pharmaceutical formulations are also provided.
Abstract:A method of producing undifferentiated avian cells expressing an embryonic stem cell phenotype is disclosed. The method comprises collecting avian cells from an avian blastoderm prior to formation of the primitive streak, then depositing the avian cells in contact with a mouse fibroblast feeder cell layer, and then growing the avian cells on the mouse fibroblast feeder cell layer in the presence of a media containing leukemia inhibitory factor in a differentiation-inhibiting amount for a time sufficient to produce a sustained avian cell culture. Cell cultures produced by the aforesaid process and veterinary pharmaceutical formulations containing such cells are also disclosed.
US Patent Website:Click Here for Full Text of Patent
Title Number:US5340740
Application Number:US1992000884423
Date Filed:15/05/1992
Date Published:23/08/1994
Assignee:North Carolina State University, Raleigh, NC, USA


 
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