Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US5397706

Title:Serum-free basal and culture medium for hematopoietic and leukemia cells
Inventors:Correa, Paulo N.; Concord, Ontario, Canada
Alexrad, Arthur A.; Willowdale, Ontario, Canada
Summary:Described herein is a serum-free basal medium and methods of its use in studying the growth and differentiation of erythroid progenitor cells, other hematopoietic progenitor cells and leukemia cells. The invention relates to methods by which such cells are maintained, cultivated, grown and differentiated such that the effects of various growth factor compounds can be quantitatively evaluated. Further disclosed are properties of the medium for hematopoietic growth and differentiation which is described as the basal serum-free medium to which has been added at least one growth-promoting agent selected from heme or hemin, interleukin-3 and recombinant human stem cell factor in combination with at least one cell differentiation and promoting agent selected from erythropoietin (Epo), insulin-like growth factor (IGF) and a retinoid. Various therapeutic and experimental applications are included.
Abstract:A basal serum-free medium and a hematopoietic cell growth and differentiation-promoting serum-free medium based thereon are provided for the maintenance, cultivation, growth and differentiation of erythroid progenitor cells, other hematopoietic progenitor cells, and leukemia cells in which the effects of various growth factor compounds can be quantitatively evaluated. Both media are wholly serum-free and contain no intrinsic growth factor compounds. The hematopoietic growth and differentiation medium consists essentially of the basal serum-free medium to which has been added at least one primarily but not exclusively growth-promoting agent selected from heme or hemin, interleukin-3 and recombinant human stem cell factor (and optimally all of them), and at least one primarily but not exclusively cell differentiation -promoting agent selected from erythropoietin (Epo), insulin-like growth factor (IGF) and a retinoid (and optimally including all of them).
US Patent Website:Click Here for Full Text of Patent
Title Number:US5397706
Application Number:US1993000103419
Date Filed:06/08/1993
Date Published:14/03/1995
Assignee:None


 
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