Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US5409813

Title:Method for mammalian cell separation from a mixture of cell populations
Inventors:Schwartz, Richard M.; San Mateo, CA, USA
Summary:Described herein is a process of selectively separating at least one mammalian target cell population from a suspension of a mixture of cell populations via the use of a magnetically stabilized and fluidized bed which contains a substance with an affinity for the target cells. The invention relates to a method for both the positive and negative selection of mammalian cell populations from a mixture according to a process by which a column containing a bed of magnetizable particles with an affinity for the target cell population is fluidized with a radially and axially uniform magnetic field through which the suspension is passed which results in the binding of the particles to the target cell population which may then be collected. A variety of therapeutic and experimental applications are included.
Abstract:The invention provides a method for both the positive and negative selection of at least one mammalian cell population from a mixture of cell populations utilizing a magnetically stabilized fluidized bed. One desirable application of this method is the separation and purification of mammalian hematopoietic cells. Target cell populations include human stem cells.
US Patent Website:Click Here for Full Text of Patent
Title Number:US5409813
Application Number:US1993000130094
Date Filed:30/09/1993
Date Published:25/04/1995
Assignee:SyStemix, Inc., Palo Alto, CA, USA


 
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