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Stem Cell Related Patent Number US5409825
| Title: | Expansion of human hematopoietic progenitor cells in a liquid medium | | Inventors: | Hoffman, Ronald; Indianapolis, IN, USA
Brandt, John; Indianapolis, IN, USA | | Summary: | Described herein are methods of maintaining and expanding mammalian bone marrow cells in a serum-free medium containing a specific mixture of cytokines but essentially free of stromal cells. The invention relates to a process for supporting hematopoietic progenitor cells in a culture medium comprised of the cytokine mast cell growth factor (MGF) wherein the human hematopoietic stem cells are characterized as being CD34+ DR- CD15- and are grown in the presence of at least one cytokine such as interleukin 3 (IL-3) in combination with a granulocyte macrophage colony stimulating factor (GM-CSF). Claims of the invention include an increase in the cell number of the population as high as 20,000 fold when expanded according to this method over a period of 26 days. Therapeutic applications of such cells are included for use in transplantation. | | Abstract: | | | US Patent Website: | Click Here for Full Text of Patent | | Title Number: | US5409825 | | Application Number: | US1993000133093 | | Date Filed: | 12/10/1993 | | Date Published: | 25/04/1995 | | Assignee: | None |
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