Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US5409825

Title:Expansion of human hematopoietic progenitor cells in a liquid medium
Inventors:Hoffman, Ronald; Indianapolis, IN, USA
Brandt, John; Indianapolis, IN, USA
Summary:Described herein are methods of maintaining and expanding mammalian bone marrow cells in a serum-free medium containing a specific mixture of cytokines but essentially free of stromal cells. The invention relates to a process for supporting hematopoietic progenitor cells in a culture medium comprised of the cytokine mast cell growth factor (MGF) wherein the human hematopoietic stem cells are characterized as being CD34+ DR- CD15- and are grown in the presence of at least one cytokine such as interleukin 3 (IL-3) in combination with a granulocyte macrophage colony stimulating factor (GM-CSF). Claims of the invention include an increase in the cell number of the population as high as 20,000 fold when expanded according to this method over a period of 26 days. Therapeutic applications of such cells are included for use in transplantation.
Abstract:
US Patent Website:Click Here for Full Text of Patent
Title Number:US5409825
Application Number:US1993000133093
Date Filed:12/10/1993
Date Published:25/04/1995
Assignee:None


 
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