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Stem Cell Related Patent Number US5416260
| Title: | Homologous recombination for universal donor cells and chimeric mammalian hosts | | Inventors: | Koller, Beverly H.; Carrboro, NC, USA
Smithies, Oliver; Chapel Hill, NC, USA | | Summary: | Described herein are chimeric mice lacking Class I MHC antigens, methods and uses thereof. The invention relates to techniques by which the ?2 -microglobulin gene is inactivated through homologous recombination in embryonic stem cells and transforming embryos, such that the gene associated with MHC antigens is “knocked out”, resulting in the reduction or elimination of Class I MHC antigens in the mouse. Claims of the invention include use of the resulting cells as universal donor cells. Further disclosed are embryonic stem cells which may be modified by homologous recombination for use in producing chimeric or transgenic mammalian hosts, which in turn may be used as a source of universal donor organs. | | Abstract: | Homologous recombination is employed to inactivate genes, particularly genes associated with MHC antigens. Particularly, the .beta..sub.2 -microglobulin gene is inactivated for reducing or eliminating Class I MHC antigens. The resulting cells may be used as universal donors. In addition, embryonic stem cells may be modified by homologous recombination for use in producing chimeric or transgenic mammalian hosts, which may be used as source of universal donor organs. | | US Patent Website: | Click Here for Full Text of Patent | | Title Number: | US5416260 | | Application Number: | US1990000611020 | | Date Filed: | 09/11/1990 | | Date Published: | 16/05/1995 | | Assignee: | University of North Carolina at Chapel Hill, Chapel Hill, NC, USA |
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