Methods comprising apoptosis inhibitors for the generation of transgenic pigs

Stem Cell Clinic
Patient Application
FAQ
Contact
Locations
News
Videos
Research
Patents
3D Culture/Scaffold
Administration
Differentiation
Expansion
Extraction/Preservation
Mobilization
Type
USE
 


Stem Cell Related Patent Number US5416260

Title:Homologous recombination for universal donor cells and chimeric mammalian hosts
Inventors:Koller, Beverly H.; Carrboro, NC, USA
Smithies, Oliver; Chapel Hill, NC, USA
Summary:Described herein are chimeric mice lacking Class I MHC antigens, methods and uses thereof. The invention relates to techniques by which the ?2 -microglobulin gene is inactivated through homologous recombination in embryonic stem cells and transforming embryos, such that the gene associated with MHC antigens is “knocked out”, resulting in the reduction or elimination of Class I MHC antigens in the mouse. Claims of the invention include use of the resulting cells as universal donor cells. Further disclosed are embryonic stem cells which may be modified by homologous recombination for use in producing chimeric or transgenic mammalian hosts, which in turn may be used as a source of universal donor organs.
Abstract:Homologous recombination is employed to inactivate genes, particularly genes associated with MHC antigens. Particularly, the .beta..sub.2 -microglobulin gene is inactivated for reducing or eliminating Class I MHC antigens. The resulting cells may be used as universal donors. In addition, embryonic stem cells may be modified by homologous recombination for use in producing chimeric or transgenic mammalian hosts, which may be used as source of universal donor organs.
US Patent Website:Click Here for Full Text of Patent
Title Number:US5416260
Application Number:US1990000611020
Date Filed:09/11/1990
Date Published:16/05/1995
Assignee:University of North Carolina at Chapel Hill, Chapel Hill, NC, USA


 
Copyright © 2007 The Institute for Cellular Medicine  6/13/2021