Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US5423778

Title:System and method for transplantation of cells
Inventors:Eriksson, Elof; Wellesley Hills, MA, USA
Vogt, Peter M.; Newton, MA, USA
Summary:Described herein is a method of culturing cells at a specific site on a patient. The invention relates to the transfer of genetic material with viral vectors or a plasmid in combination with a wound treatment chamber into keratinocytes, such as those which include a high percentage of epidermal stem cells. The treatment chamber is further described as formed on a flexible material that is impermeable to moisture and gas and which covers the site. Further claims of the invention include improved efficacy as a means of implanting genetically engineered cells and obtaining long term survival. Also disclosed are methods by which the wound chamber system may be utilized to direct in vivo gene transfer to exposed cells in an open wound, and methods by which the system also allows for the non-invasive assessment of the success of transfer by assaying for the presence of the expressed protein in wound fluid. Therapeutic applications are included for the expression of a variety of proteins secreted into the general blood and lymphatic system, or for altering the properties of a protein, such as to inhibit the expression of an immune response against a transplanted cell.
Abstract:Gene transfer of genetic material with viral vectors or plasmid, in combination with a wound treatment chamber, into keratinocytes, especially those including a high percentage of epidermal stem cells, has been demonstrated to be particularly effective as a means of implanting genetically engineered cells and obtaining long term survival. By employing the wound chamber system, direct in vivo gene transfer can also be done to exposed cells in an open wound. Skin stem cells which are located in the hair follicles are used to greatly enhance long term survival. The use of the wound chamber system for gene transfer also allows non-invasive assessment of the success of transfer by assaying for the presence of the expressed protein in wound fluid, in contrast to the prior art use of invasive techniques, such as biopsies, in order to achieve the same assessment of early expression. A wide variety of proteins and materials can be expressed, either for secretion into the general blood and lymphatic system, or to alter the properties of the protein, for example, to not express proteins eliciting an immune response against the transplanted cell.
US Patent Website:Click Here for Full Text of Patent
Title Number:US5423778
Application Number:US1992000897357
Date Filed:11/06/1992
Date Published:13/06/1995
Assignee:Eriksson; Elof, Wellesley Hills, MA, USA


 
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