Methods comprising apoptosis inhibitors for the generation of transgenic pigs

Stem Cell Clinic
Patient Application
FAQ
Contact
Locations
News
Videos
Research
Patents
3D Culture/Scaffold
Administration
Differentiation
Expansion
Extraction/Preservation
Mobilization
Type
USE
 


Stem Cell Related Patent Number US5429938

Title:Methods and compositions for isolation and growth of kidney tubule stem cells, in vitro kidney tubulogenesis and ex vivo construction of renal tubules
Inventors:Humes, H. David; Ann Arbor, MI, USA
Summary:Described herein are methods, compositions and applications of a renal cell culture. The invention relates to culture media conditions which provide for the isolation and purification of renal tubule stem cells and for in vitro kidney tubulogenesis, such that adult kidney cells are cultured in media that have been treated with combinations of transforming growth factor-?1, epidermal growth factor, and all-trans retinoic acid. Further disclosed are properties of the tubulogenesis which is described as a phenotypic transformation of the cells such that condensed aggregates of tubule cells form about a central lumen which is bordered by cells possessing a polarized epithelial phenotype with extensive microvilli formation and tight junctional complexes along the lumenal border. Therapeutic applications in the regeneration of renal tissue are provided.
Abstract:Methods, including culture media conditions, which provide for isolation and purification of renal tubule stem cells and for in vitro kidney tubulogenesis are disclosed. The methods rely on culturing adult kidney cells in a culture media treated with combinations of transforming growth factor-.beta..sub.1, epidermal growth factor, and all-trans retinoic acid.
US Patent Website:Click Here for Full Text of Patent
Title Number:US5429938
Application Number:US1992000844758
Date Filed:02/03/1992
Date Published:04/07/1995
Assignee:University of Michigan, Ann Arbor, MI, USA


 
Copyright © 2007 The Institute for Cellular Medicine  6/13/2021