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Stem Cell Related Patent Number US5429938
| Title: | Methods and compositions for isolation and growth of kidney tubule stem cells, in vitro kidney tubulogenesis and ex vivo construction of renal tubules | | Inventors: | Humes, H. David; Ann Arbor, MI, USA | | Summary: | Described herein are methods, compositions and applications of a renal cell culture. The invention relates to culture media conditions which provide for the isolation and purification of renal tubule stem cells and for in vitro kidney tubulogenesis, such that adult kidney cells are cultured in media that have been treated with combinations of transforming growth factor-?1, epidermal growth factor, and all-trans retinoic acid. Further disclosed are properties of the tubulogenesis which is described as a phenotypic transformation of the cells such that condensed aggregates of tubule cells form about a central lumen which is bordered by cells possessing a polarized epithelial phenotype with extensive microvilli formation and tight junctional complexes along the lumenal border. Therapeutic applications in the regeneration of renal tissue are provided. | | Abstract: | Methods, including culture media conditions, which provide for isolation and purification of renal tubule stem cells and for in vitro kidney tubulogenesis are disclosed. The methods rely on culturing adult kidney cells in a culture media treated with combinations of transforming growth factor-.beta..sub.1, epidermal growth factor, and all-trans retinoic acid. | | US Patent Website: | Click Here for Full Text of Patent | | Title Number: | US5429938 | | Application Number: | US1992000844758 | | Date Filed: | 02/03/1992 | | Date Published: | 04/07/1995 | | Assignee: | University of Michigan, Ann Arbor, MI, USA |
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