Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US5436151

Title:Method for culturing human hematopoietic stem cells in vitro
Inventors:McGlave, Philip B.; St. Paul, MN, USA
Verfaillie, Catherine M.; St. Paul, MN, USA
Miller, Jeffrey S.; Little Canada
, MN, USA
Summary:Described herein are methods of culturing hematopoietic cells, and applications thereof. The invention relates to a process by which hematopoietic stem cells are cultured in a non-contacting system with stromal cells whereby differentiation and replication are maintained for prolonged periods of time. Further described are methods by which the populations are preferably maintained in a liquid stromal growth medium, which extends the ability of the stem cells in the population to differentiate and self-replicate for extended periods. The population of cells is described herein as comprising an effective stimulatory amount of IL-3 and the macrophage inflammatory protein-1?a (MIP-1?a). Therapeutic applications to a variety of human diseases are included. This invention represents a continuation of other applications filed by the same assignee and inventors.
Abstract:A method for culturing mammalian hematopoietic stem cells is provided comprising maintaining a population of human stem cells in fixed, non-contacting relationship to a population of cultured stromal cells, which populations are in liquid stromal growth medium connection, so that the ability of the stem cells to differentiate and self-replicate is maintained during an extended culture period.
US Patent Website:Click Here for Full Text of Patent
Title Number:US5436151
Application Number:US1992000862814
Date Filed:03/04/1992
Date Published:25/07/1995
Assignee:Regents of the University of Minnesota, Minneapolis, MN, USA


 
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