Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US5437994

Title:Method for the ex vivo replication of stem cells, for the optimization of hematopoietic progenitor cell cultures, and for increasing the metabolism, GM-CSF secretion and/or IL-6 secretion of human stromal cells
Inventors:Emerson, Stephen G.; Ann Arbor, MI, USA
Clarke, Michael F.; Ann Arbor, MI, USA
Palsson, Bernhard O.; Ann Arbor, MI, USA
Summary:This invention describes a method for the culturing of human stem cells using a high culture medium exchange rate with the removal of metabolic byproducts and the replenishing of depleted nutrients. Described herein are culture media conditions which provide for ex vivo human stem cell division and the optimization of human hematopoietic progenitor cell cultures as well as an increase in the metabolism, and of the GM-CSF secretion or of the IL-6 secretion, of human stromal cells. Further disclosed are methods by which a liquid culture medium is preferably perfused, either continuously or periodically, at a replacement rate of 50 to 100% per day, so that metabolic byproducts are removed while depleted nutrients are replenished as the culture is maintained under physiologically acceptable conditions in the presence of growth factors. This invention represents a continuation of other applications filed by the same assignee.
Abstract:Methods, including culture media conditions, which provide for ex vivo human stem cell division and/or the optimization of human hematopoietic progenitor cell cultures and/or increasing the metabolism or GM-CSF secretion or IL-6 secretion of human stromal cells are disclosed. The methods rely on culturing human stem cells and/or human hematopoietic progenitor cells and/or human stromal cells in a liquid culture medium which is replaced, preferably perfused, either continuously or periodically, at a rate of 1 ml of medium per ml of culture per about 24 to about 48 hour period, and removing metabolic products and replenishing depleted nutrients while maintaining the culture under physiologically acceptable conditions. Optionally growth factors are added to the culture medium.
US Patent Website:Click Here for Full Text of Patent
Title Number:US5437994
Application Number:US1993000164779
Date Filed:10/12/1993
Date Published:01/08/1995
Assignee:Regents of the University of Michigan, Ann Arbor, MI, USA


 
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