Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US5453357

Title:Pluripotential embryonic stem cells and methods of making same
Inventors:Hogan, Brigid L. M.; Brentwood, TN, USA
Summary:This invention describes a method of producing pluripotent embryonic stem cells, and properties thereof. Described herein is a process by which non-mouse pluripotent stem cells may be maintained on feeder layers for at least 20 passages from which they give rise to embryoid bodies and multiple differentiated cell phenotypes. Further disclosed are properties of the monolayer culture which is described as a medium containing a fibroblast growth factor, a leukemia inhibitory factor, a membrane- associated steel factor and a soluble steel factor to primordial germ cells
Abstract:The present invention provides a non-mouse pluripotential embryonic stem cell which can: (a) be maintained on feeder layers for at least 20 passages; and (b) give rise to embryoid bodies and multiple differentiated cell phenotypes in monolayer culture. The invention further provides a method of making a pluripotential embryonic stem cell comprising administering a growth enhancing amount of basic fibroblast growth factor, leukemia inhibitory factor, membrane associated steel factor, and soluble steel factor to primordial germ cells under cell growth conditions, thereby making a pluripotential embryonic stem cell.
US Patent Website:Click Here for Full Text of Patent
Title Number:US5453357
Application Number:US1992000958562
Date Filed:08/10/1992
Date Published:26/09/1995
Assignee:Vanderbilt University, Nashville, TN, USA


 
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