Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US5459069

Title:Device for maintaining and growing human stem and/or hematopoietics cells
Inventors:Palsson, Bernhard O.; Ann Arbor, MI, USA
Emerson, Stephen G.; Ann Arbor, MI, USA
Schwartz, Richard M.; Ann Arbor, MI, USA
Summary:This invention introduces a bioreactor and methods for its use in the growth of human stem and hematopoietic cells in culture. Described herein is a device consisting of a culture chamber through which culture media and respiratory cells are circulated such that diverse cell types are simultaneously cultured in the presence of appropriate levels of nutrients and growth factors that are continuously maintained while undesirable metabolic byproducts are removed. Claims of the invention include the successful ex vivo reconstruction of hematopoietic tissue from this simultaneous culturing of multiple cell types. Further disclosed are methods by which at least one growth factor is provided through excretion by transfected stromal cells such as heterologous cells such that stromal and hematopoietic cells are maintained separately so that both the adherent and non-adherent cells are harvested. This invention represents a continuation of other applications filed by the same assignee.
Abstract:Devices are provided for the growth of human stem and/or hematopoietic cells in culture. Bioreactors are provided in which diverse cell types are simultaneously-cultured in the presence of appropriate levels of nutrients and growth factors substantially continuously maintained in the bioreactor while removing undesirable metabolic products. This simultaneous culture of multiple cell types successfully reconstructs hematopoietic tissue ex vivo. Optionally, at least one growth factor is provided through excretion by transfected stromal cells, particularly heterologous cells. The stromal cells and hematopoietic cells are maintained separately and both the adherent and non-adherent cells are harvested.
US Patent Website:Click Here for Full Text of Patent
Title Number:US5459069
Application Number:US1994000178433
Date Filed:06/01/1994
Date Published:17/10/1995
Assignee:The Regents of the University of Michigan, Ann Arbor, MI, USA


 
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