Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US5474687

Title:Methods for enriching CD34.sup.+ human hematopoietic progenitor cells
Inventors:Van Vlasselaer, Peter; Sunnyvale, CA, USA
Summary:Described herein is a process of rapidly enriching CD34+ cells from a cell mixture by means of centrifugation and a density gradient solution. The invention relates to methods of enriching hematopoietic progenitor cells from body fluids by the use of a cell-trap centrifugation tube containing a gradient solution which is adjusted to a specific density to enrich CD34+ cells from apheresed blood. Further disclosed is the configuration of the apparatus such that a tube allows the desired cell population to be collected by decantation after centrifugation thereby minimizing cell loss. Additional claims of the invention include simplification by density-adjusted cell sorting according to which cell type-specific binding agents are utilized such as antibodies and lectins that are linked to carrier particles thereby imparting a different density to undesired cell populations and allowing the progenitor cells to be separated during centrifugation. Therapeutic applications are included for the use of such donor cell preparations in bone marrow transplantation without the need for invasive procedures such as bone marrow aspiration.
Abstract:The present invention relates to methods of enriching hematopoietic progenitor cells from body fluids. In particular, it relates to the use of a cell-trap centrifugation tube containing a gradient solution adjusted to a specific density to enrich for CD34.sup.+ cells from apheresed blood. The tube allows the desired cell population to be collected by decantation after centrifugation to minimize cell loss and maximize efficiency. In addition, the method can be further simplified by density-adjusted cell sorting which uses cell type-specific binding agents such as antibodies and lectins linked to carrier particles to impart a different density to undesired cell populations allowing the progenitor cells to be separated during centrifugation in a more convenient manner. The rapid progenitor cell enrichment method described herein has a wide range of applications, including but not limited to, donor cell preparation for bone marrow transplantation without the use of invasive procedures such as bone marrow aspiration.
US Patent Website:Click Here for Full Text of Patent
Title Number:US5474687
Application Number:US1994000299469
Date Filed:31/08/1994
Date Published:12/12/1995
Assignee:Activated Cell Therapy, Inc., Mountain View, CA, USA


 
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