Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US5525708

Title:Covalent dimer of kit ligand
Inventors:Nocka, Karl H.; Harvard, MA, USA
Lobell, Robert B.; Watertown, MA, USA
Summary:This invention describes a method of preparing a modified form of kit ligand, wherein the protein has been stabilized by an intermolecular covalent linkage. The biologically active, interchain disulfide-linked dimer of kit ligand consists of 2 monomers, each one having the same amino acid sequence, with the covalent dimer being free of such monomers and inactive dimers of kit ligand.
Abstract:A modified form of KL, the ligand for the c-Kit proto-oncogene, has been prepared wherein the protein is stabilized by an intermolecular covalent linkage. The protein can be prepared by expression of a recombinant protein which is dissolved in denaturant and refolded under conditions resulting in a disulfide linked dimer. Examples demonstrate the purification and characterization of this disulfide-linked cysteine dimer kit ligand (KL-CD) which contains at least one intermolecular disulfide bond and has at least ten-fold greater activity in promoting cell proliferation than native, non-covalently linked KL, as measured in in vitro assays.
US Patent Website:Click Here for Full Text of Patent
Title Number:US5525708
Application Number:US1994000220379
Date Filed:28/03/1994
Date Published:11/06/1996
Assignee:CytoMed, Inc., Cambridge, MA, USA


 
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