Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US5556783

Title:Methods of culturing and modulating the growth of hair follicular stem cells
Inventors:Lavker, Robert M.; Malvern, PA, USA
Sun, Tung-Tien; Scarsdale, NY, USA
Yang, Jing-Shan; New York, NY, USA
Summary:This invention describes a process of identifying and modulating the activity of label-retaining stem cells such as hair follicular stem cells. Described herein are methods for isolating a subpopulation of follicular keratinocytes from the upper portion of a hair follicle, dispersing the isolated keratinocytes into a single cell suspension, contacting the cells with a selected agent either for the purpose of stimulating growth or for killing the cells, and then growing the dispersed isolated keratinocytes in the presence of 3T3 feeder cells. Further disclosed is a method of evaluating the efficacy of agents for modulating the activity of bulge cell populations by exposing bulge cells to the agent to be tested and comparing the response of the test cells to established controls. Therapeutic applications are included for the diagnosis and treatment of abnormal hair growth and skin cancers. This invention represents a continuation of other applications filed by the same assignees.
Abstract:A method of culturing hair follicular stem cells by isolating a subpopulation of follicular keratinocytes from the upper portion of a hair follicle, dispersing the isolated keratinocytes into a single cell suspension, and growing the dispersed isolated keratinocytes in the presence of 3T3 feeder cells is provided. A method of evaluating the efficacy of agents for modulating the activity of bulge cell populations by exposing bulge cells to an agent to be tested and comparing the response of the test cells to established controls for bulge cells is also provided. Methods of modulating the activity of hair follicular stem cells by identifying a subpopulation of follicular keratinocytes from the upper portion of a hair follicle and contracting the cells with a selected agent to stimulate growth or to selectively kill the cells is also provided.
US Patent Website:Click Here for Full Text of Patent
Title Number:US5556783
Application Number:US1993000086199
Date Filed:01/07/1993
Date Published:17/09/1996
Assignee:Trustees of the University of Pennsylvania, Philadelphia, PA, USA


 
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