Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US5599703

Title:In vitro amplification/expansion of CD34.sup.+ stem and progenitor cells
Inventors:Davis, Thomas A.; Chantilly, VA, USA
Kessler, Steven; Cupertino, CA, USA
Robinson, Douglas H.; Washington, DC, USA
Summary:This invention introduces a method for the high yield in vitro amplification of stem cells in the presence of endothelial cells and cytokines. Described herein is a process by which hematopoietic CD34+ stem and progenitor cells are isolated from human bone marrow and contacted with endothelial cells, such that the resulting combination of contacted stem cells and endothelial cells is cultured in the presence of at least one cytokine in an amount sufficient to support amplification and expansion of the hematopoietic CD34+ stem and progenitor cells. Claims of the invention include an increased yield of hematopoietic CD34+ stem and progenitor cells derived according to this method. Therapeutic applications are included for use in bone marrow transplantation, blood transfusion and gene therapy.
Abstract:The present invention relates to a method of amplifying in vitro stemcells. In this method hematopoietic CD34.sup.+ stem and progenitor cells are isolated from human bone marrow and contacted with endothelial cells. The contacted stem cells and endothelial cells are cultured in the presence of at least one cytokine in an amount sufficient to support amplification/expansion of the hematopoietic CD34.sup.+ stem and progenitor cells. This method produces increased yields of hematopoietic CD34.sup.+ stem and progenitor cells which can be used in human therapeutics.
US Patent Website:Click Here for Full Text of Patent
Title Number:US5599703
Application Number:US1993000142569
Date Filed:28/10/1993
Date Published:04/02/1997
Assignee:The USA as represented by the Secretary of the Navy, Washington, DC, USA


 
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