Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US5605822

Title:Methods, compositions and devices for growing human hematopoietic cells
Inventors:Emerson, Stephen G.; Ann Arbor, MI, USA
Summary:This invention describes a system for culturing human hematopoietic cells via the use of transformed fibroblasts attached to a protein substrate which provides growth factors for the cells. Herein are described methods, materials and techniques for the growth of hematopoietic cells in culture, which includes the use of bioreactors in which diverse cell types are simultaneously cultured in the presence of appropriate levels of nutrients and growth factors which are continuously maintained in the bioreactor while undesirable metabolic products are removed. This simultaneous culturing of multiple cell types is required for the successful reconstruction of hematopoietic tissue ex vivo, as at least one growth factor is provided through excretion by transfected stromal cells, particularly heterologous cells. Further described are the means by which stromal and hematopoietic cells are maintained separately, to allow for early removal of the hematopoietic cells. Methods of proliferation and expansion are also provided.
Abstract:Methods, compositions and devices are provided for the growth of hematopoietic cells in culture. Bioreactors are provided in which diverse cell types are simultaneously cultured in the presence of appropriate levels of nutrients and growth factors substantially continuously maintained in the bioreactor while removing undesirable metabolic products. This simultaneous culture of multiple cell types is required for the successful reconstruction of hematopoietic tissue ex vivo. At least one growth factor is provided through excretion by transfected stromal cells, particularly heterologous cells. Means are provided for maintaining the stromal cells and hematopoietic cells separately, to allow for early removal of the hematopoietic cells.
US Patent Website:Click Here for Full Text of Patent
Title Number:US5605822
Application Number:US1994000352196
Date Filed:01/12/1994
Date Published:25/02/1997
Assignee:The Regents of the University of Michigan, Ann Arbor, MI, USA


 
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