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Stem Cell Related Patent Number US5605829
| Title: | Stroma-derived stem cell proteoglycan growth factor | | Inventors: | McGlave, Philip B.; St. Paul, MN, USA
Verfaillie, Catherine M.; St. Paul, MN, USA
Gupta, Pankaj; Minneapolis, MN, USA | | Summary: | This invention describes methods of cultivation of hematopoietic stem cells in media containing new synthetic proteoglycans. Further described is an anionic macromolecule containing a composition that is capable of supporting conservation and differentiation of long-term bone marrow culture-initiating cells in cultured mammalian hematopoietic cells. The composition under consideration is a mixture of glycoproteins, more specifically proteoglycans, which are of a molecular weight greater than 200 kD. Emphasis is given to a synthetic proteoglycan, having a core protein and a polysaccharide portion, which is capable of promoting differentiation and maintaining the self-renewal capacity of long-term bone marrow culture-initiating cells in cultured mammalian hematopoietic cells. The polysaccharide portion of the synthetic proteoglycan comprises heparin sulfate, chondroitin sulfate, dermatan sulfate or a combination thereof, and the core protein comprises ovalbumin. | | Abstract: | An anionic macromolecular containing composition is disclosed that is capable of supporting conservation and differentiation of long-term bone marrow culture initiating cells in cultured mammalian hematopoietic cells. The composition disclosed is a mixture of glycoproteins, more specifically proteoglycans, which are of a molecular weight greater than 200 kD. | | US Patent Website: | Click Here for Full Text of Patent | | Title Number: | US5605829 | | Application Number: | US1994000346893 | | Date Filed: | 30/11/1994 | | Date Published: | 25/02/1997 | | Assignee: | Regents of the University of Minnesota, Minneapolis, MN, USA |
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