Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US5621090

Title:Nucleic acids encoding soluble human FLK-2 extracellular domain
Inventors:Lemischka, Ihor R.; Princeton, NJ, USA
Summary:This invention describes recombinant nucleic acid molecules that encode a protein containing the extracellular domain of human flk-2 and which lack the flk-2 intracellular catalytic domain. Disclosed herein are isolated mammalian nucleic acid molecules encoding receptor protein tyrosine kinases expressed in primitive hematopoietic cells and not expressed in mature hematopoietic cells; further disclosed are properties of the receptors encoded by such nucleic acid molecules, ligands for the receptors, and nucleic acid sequences that encode the ligands. Also provided are methods of stimulating the proliferation and differentiation of primitive mammalian hematopoietic stem cells via a process of contacting the stem cells with a ligand that binds to a receptor protein tyrosine kinase. Therapeutic applications are included for primitive hematopoietic cell proliferation and for the differentiation of stimulatory ligands in the treatment of anemia. This invention represents a continuation of other applications filed by the same assignee and inventor.
Abstract:Isolated mammalian nucleic acid molecules encoding receptor protein tyrosine kinases expressed in primitive hematopoietic cells and not expressed in mature hematopoietic cells are provided. Also included are the receptors encoded by such nucleic acid molecules; the nucleic acid molecules encoding receptor protein tyrosine kinases having the sequences shown in FIG. 1A (murine flk-2), FIG. 1B (human flk-2) and FIG. 2 (murine flk-1); the receptor protein tyrosine kinases having the amino acid sequences shown in FIG. 1A, FIG. 1B and FIG. 2; ligands for the receptors; nucleic acid sequences that encode the ligands; and methods of stimulating the proliferation and/or differentiation of primitive mammalian hematopoietic stem cells comprising contacting the stem cells with a ligand that binds to a receptor protein tyrosine kinase expressed in primitive mammalian hematopoietic cells and not expressed in mature hematopoietic cells.
US Patent Website:Click Here for Full Text of Patent
Title Number:US5621090
Application Number:US1992000906397
Date Filed:26/06/1992
Date Published:15/04/1997
Assignee:The Trustees of Princeton University, Princeton, NJ, USA


 
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