Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US5635386

Title:Methods for regulating the specific lineages of cells produced in a human hematopoietic cell culture
Inventors:Palsson, Bernhard O.; Ann Arbor, MI, USA
Armstrong, R. Douglas; Ann Arbor, MI, USA
Clarke, Michael F.; Ann Arbor, MI, USA
Emerson, Stephen G.; Ann Arbor, MI, USA
Summary:Described herein are methods of culturing human stem cells in order to control lineage development. The invention relates to methods and materials by which human hematopoietic progenitor cells are expanded ex vivo in a liquid culture medium which is perfused, either continuously or periodically, at an exchange rate of 50% to 100% per day, during which time metabolic byproducts are removed and depleted nutrients are replenished while the culture is maintained under physiologically acceptable conditions in the presence of growth factors. Also provided are methods by which malignant cells or the T cell and B cell content of human hematopoietic cell populations may be depleted. Therapeutic applications are included for treatment of AIDS and related T cell diseases.
Abstract:Methods, including culture media conditions, which provide for in vitro human stem cell division and/or the optimization of human hematopoietic progenitor cell cultures and/or increasing the metabolism or GM-CSF secretion or IL-6 secretion of human stromal cells and/or a method for assaying the effect of a substance or condition on a human hematopoietic cell population, and/or depleting the malignant cell or T-cell and B-cell content of a human hematopoietic cell population are disclosed. The methods rely on culturing human stem cells and/or human hematopoietic progenitor cells and/or human stromal cells in a liquid culture medium which is replaced, preferably perfused, either continuously or periodically, at a rate of 1 ml of medium per ml of culture per about 24 to about 48 hour period, and removing metabolic products and replenishing depleted nutrients while maintaining the culture under physiologically acceptable conditions. Optionally, growth factors are added to the culture medium. The disclosed culture conditions afford improved methods for bone marrow transplantation.
US Patent Website:Click Here for Full Text of Patent
Title Number:US5635386
Application Number:US1994000334011
Date Filed:02/11/1994
Date Published:03/06/1997
Assignee:The Regents of the University of Michigan, Ann Arbor, MI, USA


 
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