Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US5637323

Title:Method of mobilizing pluripotential hematopoietic stem cells with IL-7
Inventors:Wiltrout, Robert H.; Frederick, MD, USA
Summary:This invention describes a method of mobilizing hematopoietic stem cells via the administration of interleukin-7. Described herein is a method of isolating an increased number of hematopoietic stem cells from a subject after interleukin-7 has been administered to the subject in an amount that mobilizes the hematopoietic stem cells to the peripheral blood, from which a population of leukocytes enriched for hematopoietic stem cells may be isolated. Further disclosed are methods of transplanting an increased number of hematopoietic stem cells from a donor into a recipient to enhance repopulation of the recipient's hematopoietic and immune cells, according to the protocol by which interleukin-7 is administered to the donor in an amount that mobilizes the hematopoietic stem cells to the peripheral blood, from which a population of leukocytes enriched for hematopoietic stem cells is isolated from the donor's peripheral blood and transplanted into the recipient, thereby enhancing the repopulation of the recipient's hematopoietic and immune cells. Further claims of the invention include a method of improving engraftment of a bone marrow transplant as well as of a peripheral blood leukocyte transplant from a donor to a recipient via the administration of interleukin-7 to the recipient following transplantation in an amount that enhances engraftment.
Abstract:This invention provides a method of isolating an increased number of hematopoietic stem cells from a subject comprising a) administering interleukin-7 to the subject in an amount that mobilizes the hematopoietic stem cells to the peripheral blood; and, b) isolating a population of leukocytes enriched for hematopoietic stem cells from the peripheral blood. Also provided is a method of transplanting an increased number of hematopoietic stem cells from a donor to a recipient to enhance repopulation of the recipient's hematopoietic and immune cells comprising a) administering interleukin-7 to the donor in an amount that mobilizes the hematopoietic stem cells to the peripheral blood; b) isolating a population of leukocytes enriched for hematopoietic stem cells from the donor's peripheral blood; and, c) transplanting the isolated population of leukocytes enriched for hematopoietic stem cells to the recipient, thereby enhancing the repopulation of the recipient's hematopoietic and immune cells. Finally, the invention provides a method of improving engraftment of a bone marrow transplant or a peripheral blood leukocyte transplant from a donor to a recipient comprising administering interleukin-7 to the recipient following transplantation in an amount that enhances engraftment.
US Patent Website:Click Here for Full Text of Patent
Title Number:US5637323
Application Number:US1994000341399
Date Filed:16/11/1994
Date Published:10/06/1997
Assignee:The USA as represented by the Department of Health and Human Services, Washington, DC, USA


 
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