Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US5641622

Title:Continuous centrifugation process for the separation of biological components from heterogeneous cell populations
Inventors:Lake, William C.; Laguna Niguel, CA, USA
Giesler, Richard; Deerfield, IL, USA
Epps, Dennis Van; Cary, IL, USA
Chapman, John R.; Lake Villa, IL, USA
Martinson, Jeffrey A.; Mundelein, IL, USA
Ellis, Dale R.; Wonder Lake, IL, USA
Aono, Frederick; Arlington Heights, IL, USA
Bischof, Daniel F.; McHenry, IL, USA
Summary:Described herein is a process for separating a specific cell population from a heterogeneous cell mixture in a continuous flow centrifuge. The invention relates to a method combining the techniques of immunoaffinity separation and continuous flow centrifugal separation by which a heterogeneous cell mixture is contacted to promote binding with particles to which a substance is attached that actively binds to a specific type of cell in the cell mixture. Further disclosed are properties of the binding sites of the particles which comprise a specific antibody to antigens selected from the group consisting of hematopoietic cells, tumor cells, tissue culture cell lines, antigen specific lymphocytes, bacteria, protozoa, virus particles, pathogen infected cells, rDNA transfected cells, plasma proteins, pharmaceuticals, drugs, and plant, animal and microbial toxins. Also provided are methods by which the particles are selected such that the sedimentation velocity of the particle-cell conjugate differs sufficiently from that of other cells in the cell mixture so as to allow its separation by means of a continuous flow cell separator. Claims of the invention include rapid processing of large volumes of the cell mixture with a high degree of accuracy. Therapeutic and experimental applications are included in the separation of various types of leukocytes from whole blood, bone marrow concentrate, a peripheral blood stem cell concentrate, and for the separation of precursors of lymphokine activated killer cells, tumor infiltrating lymphocyte cells, and activated killer monocytes from lymphocyte or monocyte cell concentrates or from a tissue cell preparation.
Abstract:A method combining the techniques of immunoaffinity separation and continuous flow centrifugal separation is provided for selective separation of a nucleated heterogeneous cell population from a heterogeneous cell mixture. The heterogeneous cell mixture is intimately contacted to promote binding thereto by particles having attached a substance that actively binds to a specific desired type of cell out of the cell mixture. The particles are selected so that the sedimentation velocity of the particle/cell conjugate differs sufficiently from those of other cells in the cell mixture to allow its separation by means of a continuous flow cell separator. The method rapidly processes large volumes of cell mixture with the high accuracy expected of immunoaffinity separation and can be used to separate, for example, various types of leukocytes from whole blood, bone marrow concentrate, or a peripheral blood stem cell concentrate; or precursors of lymphokine activated killer cells, tumor infiltrating lymphocyte cells, or activated killer monocytes from lymphocyte or monocyte cell concentrates or from a tissue cell preparation.
US Patent Website:Click Here for Full Text of Patent
Title Number:US5641622
Application Number:US1994000255107
Date Filed:07/06/1994
Date Published:24/06/1997
Assignee:Baxter International Inc., Deerfield, IL, USA


 
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