Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US5643741

Title:Identification and isolation of human hematopoietic stem cells
Inventors:Tsukamoto, Ann; Palo Alto, CA, USA
Baum, Charles M.; Mountain View, CA, USA
Aihara, Yukoh; Yokohama, Japan
Weissman, Irving; Palo Alto, CA, USA
Summary:This invention introduces a process for the detection and identification of human hematopoietic stem cells in a sample. Described herein are methods by which antibodies specific for the cell surface markers Thy-1 and CD34 are applied to homogeneous stem cell compositions that have been obtained by the selective isolation of cells which are free of markers associated with differentiated cells but which display epitopic characteristics associated with the stem cells. Further disclosed are methods of maintaining the cells in an appropriate growth medium, and methods of assaying such stem cells for their capacity to generate members of each of the hematopoietic lineages. Therapeutic applications are included for the use of hematopoietic stem cells derived according to this method in the treatment of a variety of diseases.
Abstract:Human hematopoietic stem cells are identified according to expression of specific cell surface markers. Substantially homogenous stem cell compositions are obtained by selective isolation of cells free of markers associated with differentiated cells, while displaying epitopic characteristics associated with the stem cells. The stem cells may then be maintained by regeneration in an appropriate growth medium. Means are provided for assaying for the stem cells as to their capability for producing members of each of the hematopoietic lineages.
US Patent Website:Click Here for Full Text of Patent
Title Number:US5643741
Application Number:US1995000466659
Date Filed:06/06/1995
Date Published:01/07/1997
Assignee:SyStemix, Inc., Palo Alto, CA, USA


 
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