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Stem Cell Related Patent Number US5646043

Title:Methods for the ex vivo replication of human stem cells and/or expansion of human progenitor cells
Inventors:Emerson, Stephen G.; Ann Arbor, MI, USA
Clarke, Michael F.; Ann Arbor, MI, USA
Palsson, Bernhard O.; Ann Arbor, MI, USA
Summary:This invention introduces a method for the culturing of human stem cells via the use of a high culture medium exchange rate coupled with the removal of metabolic byproducts and the replenishing of depleted nutrients. Described herein are methods and materials for the ex vivo replication and expansion of human hematopoietic progenitor and stromal cells in a liquid culture medium which is perfused, either continuously or periodically, at an exchange rate of 50% per day, during which time metabolic byproducts are removed and depleted nutrients are replenished while the culture is maintained under physiologically acceptable conditions in the presence of growth factors. Claims of the invention include optimized expansion of the human hematopoietic progenitor cell cultures and an increased metabolism, including increased GM-CSF secretion or IL-6 secretion, of the human stromal cells. This invention represents a continuation of other applications filed by the same assignee.
Abstract:Methods, including culture media conditions, which provide for ex vivo human stem cell division and/or the optimization of human hematopoietic progenitor cell cultures and/or increasing the metabolism or GM-CSF secretion or IL-6 secretion of human stromal cells are disclosed. The methods rely on culturing human stem cells and/or human hematopoietic progenitor cells and/or human stromal cells in a liquid culture medium which is replaced, preferably perfused, either continuously or periodically, at a rate of 1 ml of medium per ml of culture per about 24 to about 48 hour period, and removing metabolic products and replenishing depleted nutrients while maintaining the culture under physiologically acceptable conditions. Optionally growth factors are added to the culture medium.
US Patent Website:Click Here for Full Text of Patent
Title Number:US5646043
Application Number:US1995000401978
Date Filed:10/03/1995
Date Published:08/07/1997
Assignee:Regents of the University of Michigan, Ann Arbor, MI, USA

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