Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US5654183

Title:Genetically engineered mammalian neural crest stem cells
Inventors:Anderson, David J.; Altadena, CA, USA
Stemple, Derek L.; Newton, MA, USA
Summary:Described herein are genetically engineered neural crest stem cells, methods and uses thereof. The invention relates to mammalian multipotent neural stem cells and their progeny and methods for the isolation and clonal propagation of such cells which are capable of self-regeneration and asymmetrical division at the clonal level. Further disclosed are methods by which the cells are transfected with foreign nucleic acid to produce an immortalized neural stem cell, and methods by which mammalian multipotent neural stem cells may be identified from various tissue. Also provided are methods for detecting antibodies to neural cell surface markers and a monoclonal antibody to the mouse LNGFR. Therapeutic and experimental applications are included for the study of stem cell function and for the transplantation of such mammalian neural stem cells and neural or glial progenitors into mammals.
Abstract:The invention includes mammalian multipotent neural stem cells and their progeny and methods for the isolation and clonal propagation of such cells. At the clonal level the stem cells are capable of self regeneration and asymmetrical division. Lineage restriction is demonstrated within developing clones which are sensitive to the local environment. The invention also includes such cells which are transfected with foreign nucleic acid, e.g., to produce an immortalized neural stem cell. The invention further includes transplantation assays which allow for the identification of mammalian multipotent neural stem cells from various tissues and methods for transplanting mammalian neural stem cells and/or neural or glial progenitors into mammals. A novel method for detecting antibodies to neural cell surface markers is disclosed as well as a monoclonal antibody to mouse LNGFR.
US Patent Website:Click Here for Full Text of Patent
Title Number:US5654183
Application Number:US1994000188286
Date Filed:28/01/1994
Date Published:05/08/1997
Assignee:California Institute of Technology, Pasadena, CA, USA


 
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