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Stem Cell Related Patent Number US5656479

Title:Avian embryonic stem cells
Inventors:Petitte, James N.; Raleigh, NC, USA
Yang, Zengming; Raleigh, NC, USA
Summary:Described herein are cultured avian embryonic stem cells that have been obtained by growing avian blastoderm cells on a mouse fibroblast feeder cell layer in a medium containing leukemia inhibitory factor. The invention relates to a process of generating undifferentiated avian cells expressing an embryonic stem cell phenotype by collecting the avian cells from an avian blastoderm prior to the formation of the primitive streak, after which the avian cells are deposited in contact with a mouse fibroblast feeder cell layer and grown in the presence of a medium containing leukemia inhibitory factor in a differentiation-inhibiting amount for a time sufficient to produce a sustained avian cell culture. Further disclosed are various cell cultures produced according to this process, and associated veterinary pharmaceutical formulations.
Abstract:A method of producing undifferentiated avian cells expressing an embryonic stem cell phenotype is disclosed. The method comprises collecting avian cells from an avian blastoderm prior to formation of the primitive streak, then depositing the avian cells in contact with a mouse fibroblast feeder cell layer, and then growing the avian cells on the mouse fibroblast feeder cell layer in the presence of a media containing leukemia inhibitory factor in a differentiation-inhibiting amount for a time sufficient to produce a sustained avian cell culture. Cell cultures produced by the aforesaid process and veterinary pharmaceutical formulations containing such cells are also disclosed.
US Patent Website:Click Here for Full Text of Patent
Title Number:US5656479
Application Number:US1994000246610
Date Filed:20/05/1994
Date Published:12/08/1997
Assignee:North Carolina State University, Raleigh, NC, USA


 
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