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Stem Cell Related Patent Number US5663051
| Title: | Separation apparatus and method | | Inventors: | Vlasselaer, Peter Van; Sunnyvale, CA, USA | | Summary: | This invention introduces an apparatus and associated compositions for the separation of cells, comprised of a silanized silica suspension including polylactam. Described herein is an apparatus designed for enriching specific cell types from cell mixtures via a centrifugable device that includes a constriction defining a lower region and a cell separation medium, such that the constriction prevents mixing between the upper and lower portions of the device. Also provided are methods that use precisely defined cell separation media to isolate specific cells from cell mixtures, including CD34+ hematopoietic progenitor cells from blood or bone marrow, nucleated fetal cells from maternal blood, specific tumor cells, dendritic cells, natural killer cells, and natural suppressor cells from various body fluids. Further disclosed is a density adjusted cell separation technique used to augment the apparatus. Therapeutic and experimental applications are included for the prevention of cell aggregation and the cytotoxic effects of sterilization treatments. | | Abstract: | Disclosed is an apparatus designed to be used for enriching specific cell types from cell mixtures. The apparatus includes a centrifugable device that includes a constriction defining a lower region and a defined cell separation medium. The constriction prevents mixing between the upper and lower portions of the device. Also disclosed are methods that use precisely defined cell separation media to isolate specific cells from cell mixtures, including CD34.sup.+ hematopoietic progenitor cells from blood or bone marrow, nucleated fetal cells from maternal blood, specific tumor cells, dendritic cells, natural killer cells, and natural suppressor cells from various body fluids, and for enrichment or depletion of T cell lymphocytes. Also disclosed is a density adjusted cell separation technique used to augment the above apparatus and enrichment methods. The apparatus and enrichment methods are useful in various diagnostic and therapeutic regimens. | | US Patent Website: | Click Here for Full Text of Patent | | Title Number: | US5663051 | | Application Number: | US1995000570397 | | Date Filed: | 11/12/1995 | | Date Published: | 02/09/1997 | | Assignee: | Activated Cell Therapy, Inc., Mountain View, CA, USA |
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