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Stem Cell Related Patent Number US5670351

Title:Methods and compositions for the ex vivo replication of human hematopoietic stem cells
Inventors:Emerson, Stephen G.; Ann Arbor, MI, USA
Clarke, Michael F.; Ann Arbor, MI, USA
Palsson, Bernhard O.; Ann Arbor, MI, USA
Schwartz, Richard M.; Ann Arbor, MI, USA
Summary:Described herein is a method of culturing human stem cells using an efficient rate of exchange for the removal of metabolic byproducts and the replenishing of depleted nutrients. The invention relates to a process of culturing human stem, hematopoietic progenitor and stromal cells in a liquid culture medium which is preferably perfused, either continuously or periodically, during which time metabolic byproducts are removed and depleted nutrients are replenished in the presence of optimal growth factors. Several embodiments of the invention are provided. Methods and materials for the ex vivo replication of various cells are disclosed for each embodiment. This invention represents a continuation of other applications filed by the same assignee and inventors.
Abstract:Methods, including culture media conditions, which provide for ex vivo human stem cell division and stable genetic transformation and/or the optimization of human hematopoietic progenitor cell cultures and/or increasing the metabolism or GM-CSF secretion or IL-6 secretion of human stromal cells are disclosed. The methods rely on culturing human stem cells and/or human hematopoietic progenitor cells and/or human stromal cells in a liquid culture medium which is replaced, preferably perfused, either continuously or periodically, at a rate of 1 ml of medium per ml of culture per about 24 to about 48 hour period, and removing metabolic products and replenishing depleted nutrients while maintaining the culture under physiologically acceptable conditions. Optionally growth factors are added to the culture medium.
US Patent Website:Click Here for Full Text of Patent
Title Number:US5670351
Application Number:US1994000366493
Date Filed:30/12/1994
Date Published:23/09/1997
Assignee:The Regents of the University of Michigan, Ann Arbor, MI, USA

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