Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US5670372

Title:Pluripotential embryonic stem cells and methods of making same
Inventors:Hogan, Brigid L. M.; Brentwood, TN, USA
Summary:Described herein is a test for substances that promote the formation of embryonic stem cells via incubation with primordial germ cells and a growth factor. The invention relates to a method of screening factors for the ability to promote the formation of embryonic stem cells, via the use of a non-mouse pluripotent embryonic stem cell which can be maintained on feeder layers for at least 20 passages and which gives rise to embryoid bodies and multiple differentiated cell phenotypes in a monolayer culture. Further disclosed is a method of making pluripotent embryonic stem cells via the administration of a growth-enhancing amount of basic fibroblast growth factor, leukemia inhibitory factor, membrane associated steel factor, and soluble steel factor to primordial germ cells under specific cell growth conditions. Experimental applications are provided.
Abstract:The present invention provides a non-mouse pluripotential embryonic stem cell which can be maintained on feeder layers for at least 20 passages and give rise to embryoid bodies and multiple differentiated cell phenotypes in monolayer culture. The invention further provides a method of making a pluripotential embryonic stem cell comprising administering a growth enhancing amount of basic fibroblast growth factor, leukemia inhibitory factor, membrane associated steel factor, and soluble steel factor to primordial germ cells under cell growth conditions, thereby making a pluripotential embryonic stem cell.
US Patent Website:Click Here for Full Text of Patent
Title Number:US5670372
Application Number:US1995000463192
Date Filed:05/06/1995
Date Published:23/09/1997
Assignee:Vanderbilt University, USA


 
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