Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US5672346

Title:Human stem cell compositions and methods
Inventors:Srour, Edward; Indianapolis, IN, USA
Zanjani, Esmail; Reno, NV, USA
Brandt, John E.; Indianapolis, IN, USA
Hoffman, Ronald; Indianapolis, IN, USA
Summary:Described herein are human pluripotent hematopoietic stem cell (PHSC) compositions comprised of CD34+, HLA-DR- and expressing the receptor for the C-kit ligand (Krf). The invention relates to compositions enriched with human PHSCs and composed of homogeneous populations of the human hematopoietic cells which are c-kit receptor positive (KR+) and which are capable of in vitro self-renewal and differentiation to the erythroid, myeloid, and megakaryocytic lineages. Also provided are methods by which the PHSCs may be grafted into a host. Therapeutic applications in transplantation and gene therapy are provided.
Abstract:Disclosed are human pluripotent hematopoietic stem cell (PHSC) enriched compositions and methods for obtaining and using the compositions. A substantially homogeneous population of human hematopoietic cells characterized as CD34.sup.+, HLA-DR.sup.-, and c-kit receptor positive (KR.sup.+) and capable of in vitro self-renewal and differentiation to members of at least the erythroid, myeloid, and megakaryocytic lineages, is obtained by separating the population from a cellular mixture, for example by cytometric cell sorting techniques. Also disclosed is a method of obtaining persistent maintenance of grafted human hematopoietic cells in a mammal, which includes grafting a mammal in utero with a cellular composition enriched in human hematopoietic cells characterized as CD34.sup.+, HLA-DR.sup.- and capable of in vitro self-renewal and differentiation to members of the lymphoid, myeloid, erythroid and megakaryocytic lineages.
US Patent Website:Click Here for Full Text of Patent
Title Number:US5672346
Application Number:US1993000077134
Date Filed:15/06/1993
Date Published:30/09/1997
Assignee:Indiana University Foundation, Bloomington, IN, USA


 
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