Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US5672499

Title:Immoralized neural crest stem cells and methods of making
Inventors:Anderson, David J.; Altadena, CA, USA
Stemple, Derek L.; Newton, MA, USA
Summary:This invention describes a method for immortalizing neural crest stem cells via the introduction of an immortalization gene. Described herein are mammalian multipotent neural stem cells, their progeny, and methods for the isolation and clonal propagation of such cells which exhibit the capacity for self-regeneration and asymmetrical division at the clonal level. Also provided are methods by which cells are transfected with foreign nucleic acid to produce an immortalized neural stem cell. Further disclosed are transplantation assays which allow for the identification of mammalian multipotent neural stem cells from various tissue, and methods for transplanting mammalian neural stem cells and neural or glial progenitors into mammals. Claims of the invention include a novel method for detecting antibodies to neural cell surface markers as well as a monoclonal antibody to mouse LNGFR. Therapeutic and experimental applications are included in the research of neural crest stem cells and in the treatment of peripheral neurological disorders.
Abstract:The invention includes mammalian multipotent neural stem cells and their progeny and methods for the isolation and clonal propagation of such cells. At the clonal level the stem cells are capable of self regeneration and asymmetrical division. Lineage restriction is demonstrated within developing clones which are sensitive to the local environment. The invention also includes such cells which are transfected with foreign nucleic acid, e.g., to produce an immortalized neural stem cell. The invention further includes transplantation assays which allow for the identification of mammalian multipotent neural stem cells from various tissues and methods for transplanting mammalian neural stem cells and/or neural or glial progenitors into mammals. A novel method for detecting antibodies to neural cell surface markers is disclosed as well as a monoclonal antibody to mouse LNGFR.
US Patent Website:Click Here for Full Text of Patent
Title Number:US5672499
Application Number:US1995000478920
Date Filed:07/06/1995
Date Published:30/09/1997
Assignee:California Institute of Technology, Pasadena, CA, USA


 
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