Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US5686278

Title:Methods for enhanced retrovirus-mediated gene transfer
Inventors:Williams, David A.; Indianapolis, IN, USA
Patel, Vikram P.; Olney, MD, USA
Summary:This invention describes a method for increasing the frequency of transduction of viable hematopoietic cells using a replication-defective recombinant retrovirus vector. Described herein is a method which includes infecting the viable hematopoietic cells in the presence of fibronectin or fibronectin fragments which have been shown to enhance the retroviral-mediated gene transfer into the hematopoietic cells, including committed progenitors and primitive hematopoietic stem cells. Further claims of the invention include improved methods for somatic gene therapy based upon enhanced gene transfer. Therapeutic applications are included for gene therapy in the treatment of bone marrow disorders including cancer.
Abstract:A method to increase the efficiency of transduction of hematopoietic cells by retroviruses includes infecting the cells in the presence of fibronectin or fibronectin fragments. The fibronectin and fibronectin fragments significantly enhance retroviral-mediated gene transfer into the hematopoietic cells, particularly including committed progenitors and primitive hematopoietic stem cells. The invention also provides improved methods for somatic gene therapy capitalizing on the enhanced gene transfer, and hematopoietic cellular populations.
US Patent Website:Click Here for Full Text of Patent
Title Number:US5686278
Application Number:US1994000218355
Date Filed:25/03/1994
Date Published:11/11/1997
Assignee:Indiana University Foundation, Bloomington, IN, USA


 
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