Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US5707865

Title:Retroviral vectors for expression in embryonic cells
Inventors:Kohn, Donald B.; Tarzana, CA, USA
Challita, Pia M.; Sherman Oaks, CA, USA
Summary:Described herein are retroviral plasmid vectors, methods and uses thereof. The invention relates to a Maloney Murine Leukemia Virus LTR which lacks a negative control region but which includes an enhancer region obtained from a virus selected from the group consisting of Myeloproliferative Sarcoma Virus and polyoma virus, and which also contains a primer binding site obtained from a murine retrovirus. The vector is further described herein as including a nucleic acid sequence which encodes the demethylation of cytosine residues in the proviral LTR. Claims of the invention include the improved generation by such a plasmid vector of retroviral vector particles which may be expressed in embryonic cells, including embryonic stem cells. Therapeutic applications are included in gene therapy and for treatments requiring reduced levels of negative control and enhanced expression of a therapeutic agent.
Abstract:Retroviral plasmid vectors which include an enhancer region obtained from a virus selected from the group consisting of Myeloproliferative Sarcoma Virus and polyoma virus, and a primer binding site obtained from murine retrovirus. The retroviral plasmid vector does not include a negative control region. The vector also may include a nucleic acid sequence which encodes demethylation of cytosine residues in the proviral LTR. Such plasmid vectors are especially useful in the generation of retroviral vector particles which may be expressed in embryonic cells, such as embryonic stem cells.
US Patent Website:Click Here for Full Text of Patent
Title Number:US5707865
Application Number:US1994000361112
Date Filed:21/12/1994
Date Published:13/01/1998
Assignee:None


 
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