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Stem Cell Related Patent Number US5728581

Title:Method of expanding hematopoietic stem cells, reagents and bioreactors for use therein
Inventors:Schwartz, Richard Merrill; San Mateo, CA, USA
Tucker, Sean Newton; San Francisco, CA, USA
Chary, Srikanth Ranga; Fremont, CA, USA
Kuo, Suzanne Chang; Cupertino, CA, USA
Summary:Described herein is a method for expanding an enriched population of hematopoietic stem cells which is free of stromal cells. The invention relates to a system of expanding an inoculated population of hematopoietic stem cells in a stromal cell-free medium at specific initial oxygen concentrations in an expansion container with a volume of suitable medium. Further disclosed are properties of the bioreactor which has been constructed to accommodate the operational requirements of stromal-free stem cell expansion. Also provided are precise compositions of the medium. Therapeutic applications are included for the restoration of hematopoiesis after cancer treatment.
Abstract:The present invention provides methods and bioreactors for expanding stem cells in a population of cells substantially enriched in hematopoietic stem cells and substantially free of stromal cells. The method comprising the steps of inoculating the population of cells in an expansion container in a volume of suitable medium such that the cell density is at least about 5,000 cells/1 mL and at an initial oxygen concentration of less than 8%; adding an effective amount of at least one cytokine to cause stem cell expansion; culturing the cells under suitable conditions such that the cells condition the medium; increasing the oxygen concentration to about 20%; exchanging the medium at a rate which allows expansion of the stem cells; and culturing the cells under conditions such that the stem cells are expanded. The present invention also provides a bioreactor constructed to accomodate the operational requirements for stroma-free stem cell expansion.
US Patent Website:Click Here for Full Text of Patent
Title Number:US5728581
Application Number:US1995000482259
Date Filed:07/06/1995
Date Published:17/03/1998
Assignee:SyStemix, Inc., Palo Alto, CA, USA

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