Methods comprising apoptosis inhibitors for the generation of transgenic pigs |
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| Title: | Mouse with defective endotheline-1 gene function |
| Inventors: | Yazaki, Yoshio; Tokyo, Japan Kurihara, Hiroki; Tokyo, Japan Kurihara, Yukiko; Tokyo, Japan Suzuki, Hiroshi; Shizuoka, Japan Kodama, Tatsuhiko; Tokyo, Japan |
| Summary: | This invention describes a chimeric mouse deficient in endothelin-1 gene function by insertion of another gene into the endotheline-1 gene. Disclosed herein is the process for producing this genetically engineered mouse with altered hemodynamics, and details are provided for DNA fragment preparation and genetic disruption in conjunction with embryonic preparation and development, the generation and breeding of chimeric mice, and finally the production of the desired mouse from the previous steps. Further provided are recommendations for the use of such a mouse in the elucidation of the pathology and etiology of various cardiovascular diseases such as hypertension, arteriosclerosis and ischemic heart disease, and as a model for the investigation of cardiovascular diseases and for the development of corresponding therapeutic treatments. |
| Abstract: | There is provided a mouse lacking the function of the endotheline-1 gene by insertion of another gene into the endotheline-1 gene. The mouse is useful for elucidation of the pathological physiology and causes of and development of therapies for cardiovascular diseases such as hypertension, arteriosclerosis and ischemic heart disease. |
| US Patent Website: | Click Here for Full Text of Patent |
| Title Number: | US5750825 |
| Application Number: | US1995000325181 |
| Date Filed: | 24/04/1995 |
| Date Published: | 12/05/1998 |
| Assignee: | Chugai Seiyaku Kabushiki Kaisha, Tokyo, Japan |