Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US5753506

Title:Isolation propagation and directed differentiation of stem cells from embryonic and adult central nervous system of mammals
Inventors:Johe, Karl K.; Potomac, MD, USA
Summary:This invention describes a method for the in vitro expansion, propagation and long term culture of central nervous system stem cells. Described herein is an in vitro procedure by which a homogeneous population of multipotent precursor cells from mammalian embryonic neuroepithelium CNS stem cells can be expanded up to 109 fold in culture while retaining the capacity to differentiate into neurons, oligodendrocytes, and astrocytes. Further disclosed are methods by which a large-scale composition of the mammalian CNS stem cells, neurons, astrocytes, and oligodendrocytes may be prepared under chemically defined conditions. Therapeutic applications are included in the screening of growth factors and drugs, and in gene and cell therapy for the treatment of neurological disorders.
Abstract:The present invention reveals an in vitro procedure by which a homogeneous population of multipotential precursor cells from mammalian embryonic neuroepithelium (CNS stem cells) can be expanded up to 10.sup.9 fold in culture while maintaining their multipotential capacity to differentiate into neurons, oligodendrocytes, and astrocytes. Chemically defined conditions are presented that enable a large number of neurons, up to 50% of the expanded cells, to be derived from the stem cells. In addition, four factors--PDGF, CNTF, LIF, and T3--have been identified which, individually, generate significantly higher proportions of neurons, astrocytes, or oligodendrocytes. These defined procedures permit a large-scale preparation of the mammalian CNS stem cells, neurons, astrocytes, and oligodendrocytes under chemically defined conditions with efficiency and control. These cells should be an important tool for many cell- and gene-based therapies for neurological disorders.
US Patent Website:Click Here for Full Text of Patent
Title Number:US5753506
Application Number:US1996000719450
Date Filed:25/09/1996
Date Published:19/05/1998
Assignee:CNS Stem Cell Technology, Inc., Bethesda, MD, USA


 
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