Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US5763266

Title:Methods, compositions and devices for maintaining and growing human stem and/or hematopoietics cells
Inventors:Palsson, Bernhard O.; Ann Arbor, MI, USA
Emerson, Stephen G.; Wayne, PA, USA
Schwartz, Richard M.; San Mateo, CA, USA
Summary:Described herein is a bioreactor for growing human stem and hematopoietic cells, and which is characterized by a culture chamber through which a culture medium and respiratory cells are circulated. The invention relates to methods, compositions and devices for the growth of human stem and hematopoietic cells in culture in a bioreactor in which diverse cell types are cultured simultaneously in the presence of appropriate levels of nutrients and growth factors which are continuously maintained in the bioreactor while undesirable metabolic byproducts are removed. Claims of the invention include successfully reconstructed hematopoietic tissue ex vivo as a result of the simultaneous culture of multiple cell types. Further provided are methods by which at least one growth factor may be provided through excretion by transfected stromal cells such as heterologous cells, and methods by which stromal and hematopoietic cells may be maintained separately while both the adherent and non-adherent cells are harvested. This invention represents a continuation of other applications filed by the same assignee and inventors.
Abstract:Methods, compositions and devices are provided for the growth of human stem and/or hematopoietic cells in culture. Bioreactors are provided in which diverse cell types are simultaneously-cultured in the presence of appropriate levels of nutrients and growth factors substantially continuously maintained in the bioreactor while removing undesirable metabolic products. This simultaneous culture of multiple cell types successfully reconstructs hematopoietic tissue ex vivo. Optionally, at least one growth factor is provided through excretion by transfected stromal cells, particularly heterologous cells. The stromal cells and hematopoietic cells may be maintained separately, and both the adherent and non-adherent cells harvested.
US Patent Website:Click Here for Full Text of Patent
Title Number:US5763266
Application Number:US1994000290773
Date Filed:07/11/1994
Date Published:09/06/1998
Assignee:The Regents of the University of Michigan, Ann Arbor, MI, USA


 
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