Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US5821235

Title:Gene therapy using the intestine
Inventors:Henning, Susan June; Houston, TX, USA
Ledley, Fred D.; Houston, TX, USA
Summary:This invention describes a method of gene therapy using the intestine. Described herein is a process for the in vivo introduction of a nucleic acid cassette into stem cells of intestinal epithelium by a vector solution which may be delivered through an insertion device such as an endoscope, through catheters, through ligating and clamping of the intestine after laparotomy or through slow release capsules. Further disclosed are methods by which the vector solution is allowed to contact the stem cells of the intestinal epithelium for an amount of time sufficient to allow incorporation, which is usually between 1 and 48 hours, after which the insertion device or clamping and ligation procedure blockages are removed. Therapeutic applications are included for the treatment of a variety of diseases including metabolic, endocrine and circulatory diseases, coagulation disorders, cancer, and gastrointestinal disease. This invention represents a continuation of other applications filed by the same assignee and inventors.
Abstract:Method for in vivo introduction of a nucleic acid cassette into stem cells of intestinal epithelium. The nucleic acid cassette is introduced via vector solution. The vector solution can be delivered via the intestinal lumen in a variety of ways, including through an insertion device such as an endoscope, through catheters, through ligating and clamping the intestine after laparotomy or through slow release capsules. The vector solution once introduced into the intestinal epithelium is allowed to contact the stem cells for sufficient time for incorporation, usually between 1 and 48 hours. After sufficient incorporation, the insertion device and/or clamping and ligation procedure blockage are removed. Preferably, the procedure includes sufficient fluid to distend the intestine and provide additional access to the stem cells and the crypts. The procedure is useful in treating a variety of diseases including metabolic disorders, endocrine disorders, circulatory disorders, coagulation disorders, cancer, and gastrointestinal disease.
US Patent Website:Click Here for Full Text of Patent
Title Number:US5821235
Application Number:US1995000376473
Date Filed:20/01/1995
Date Published:13/10/1998
Assignee:Baylor College of Medicine, Houston, TX, USA


 
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