Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US5824304

Title:Peripheralization of hematopoietic stem cells
Inventors:Papayannopoulou, Thalia; Seattle, WA, USA
Summary:Described herein is a chemotherapeutic and radiotherapeutic method of treatment combined with the restoration of hematopoietic stem cells enriched from peripheral blood after treatment with the anti-VLA-4 antibody. The invention relates to methods for peripheralizing CD34+ cells, including hematopoietic stem cells, according to a process by which an anti-VLA-4 antibody is administered to a patient in order to block the binding of the VLA-4 antigen on the surface of CD34+ cells to VCAM or fibronectin, after which the peripheral blood containing the CD34+ cells is collected by leukapheresis, after which the CD34+ cells are enriched by immunoadsorption using anti-CD34 antibodies, during which time chemotherapy and/or radiotherapy are administered to the patient, after which the enriched CD34+ cells are returned to the patient's circulating blood. The invention constitutes a novel approach in the treatment of cancer and acquired immune deficiency syndrome by gene therapy.
Abstract:The invention provides methods for peripheralizing CD34.sup.+ cells, including hematopoietic stem cells. In a first aspect, the method comprises the step of administering a blocking agent of VLA-4 antigen on the surface of CD34.sup.+ cells. In a second aspect, the method comprises administering a blocking agent of VLA-4 antigen on the surface of CD34.sup.+ cells and administering a stimulating agent of CD34.sup.+ cell proliferation in vivo. The method according to the invention is useful in the treatment of cancer or AIDS, and in gene therapy.
US Patent Website:Click Here for Full Text of Patent
Title Number:US5824304
Application Number:US1995000463298
Date Filed:05/06/1995
Date Published:20/10/1998

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