Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US5834256

Title:Method for production of high titer virus and high efficiency retroviral mediated transduction of mammalian cells
Inventors:Finer, Mitchell H.; San Carlos, CA, USA
Roberts, Margo R.; San Francisco, CA, USA
Dull, Thomas J.; San Francisco, CA, USA
Zsebo, Krisztina M.; Woodside, CA, USA
Qin, Lu; Foster City, CA, USA
Farson, Deborah A.; Oakland, CA, USA
Summary:Described herein is a novel method of transducing mammalian target cells with foreign genes. The invention relates to a packaging plasmid and retroviral vector encoding such genes to produce replication-defective vectors in cells, such that the retroviral packaging constructs and packagable vector transcripts are produced from high expression plasmids by transfection in human cells. Further disclosed are methods by which high titers of recombinant retrovirus are produced in the infected cells, as are properties of novel retroviral constructs which transduce primary human cells, including T cells and bone marrow stem cells, with foreign genes by cocultivation. Claims of the invention include a rapid production of high viral supernatants, in addition to a high efficiency of transduction such that the cells are refractory to transduction by conventional means.
Abstract:The invention provides a novel retroviral packaging system, in which retroviral packaging constructs and packagable vector transcripts are produced from high expression plasmids by transfection in human cells. High titers of recombinant retrovirus are produced in infected cells. The methods of the invention include the use of the novel retroviral constructs to transduce primary human cells, including T cells and bone marrow stem cells, with foreign genes by cocultivation at high efficiencies. The invention is useful for the rapid production of high viral supernatants, and to transduce with high efficiency cells that are refractory to transduction by conventional means.
US Patent Website:Click Here for Full Text of Patent
Title Number:US5834256
Application Number:US1993000076299
Date Filed:11/06/1993
Date Published:10/11/1998
Assignee:Cell Genesys, Inc., Foster City, CA, USA


 
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