Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US5843780

Title:Primate embryonic stem cells
Inventors:Thomson, James A.; Madison, WI, USA
Summary:Described herein is a purified preparation of primate embryonic stem cells which exhibit normal karyotypes and which continue to proliferate in an undifferentiated state for extended periods when cultured on a fibroblast feeder layer. The invention relates to a preparation of cells characterized by the cell surface markers SSEA-1 (-), SSEA-3 (+), SSEA-4 (+), TRA-1-60 (+), TRA-1-81 (+), and alkaline phosphatase (+), such that cells of the preparation have normal karyotypes and continue to proliferate in an undifferentiated state after continuous culture for eleven months, while retaining the ability to form trophoblasts and to differentiate into all tissues derived from all three embryonic germ layers, namely, endoderm, mesoderm and ectoderm. Therapeutic applications to the treatment of a variety of human disease are included.
Abstract:A purified preparation of primate embryonic stem cells is disclosed. This preparation is characterized by the following cell surface markers: SSEA-1 (-); SSEA-3 (+); SSEA-4 (+); TRA-1-60 (+); TRA-1-81 (+); and alkaline phosphatase (+). In a particularly advantageous embodiment, the cells of the preparation have normal karyotypes and continue to proliferate in an undifferentiated state after continuous culture for eleven months. The embryonic stem cell lines also retain the ability, throughout the culture, to form trophoblast and to differentiate into all tissues derived from all three embryonic germ layers (endoderm, mesoderm and ectoderm). A method for isolating a primate embryonic stem cell line is also disclosed.
US Patent Website:Click Here for Full Text of Patent
Title Number:US5843780
Application Number:US1996000591246
Date Filed:18/01/1996
Date Published:01/12/1998
Assignee:Wisconsin Alumni Research Foundation, Madison, WI, USA


 
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