Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US5849553

Title:Mammalian multipotent neural stem cells
Inventors:Anderson, David J.; Altadena, CA, USA
Stemple, Derek L.; Newton, MA, USA
Summary:This invention describes a method of producing an immortalized cell line via a process of transforming neural crest stem cells with a vector containing an immortalized gene flanked by recombinase target sites. Described herein are mammalian multipotent neural stem cells and their progeny, as well as methods for the isolation and clonal propagation of such cells. Further disclosed are transplantation assays which allow for the identification of mammalian multipotent neural stem cells from various tissue, as are therapeutic applications to the transplantation of such neural or glial progenitors into humans.
Abstract:The invention includes mammalian multipotent neural stem cells and their progeny and methods for the isolation and clonal propagation of such cells. At the clonal level the stem cells are capable of self regeneration and asymmetrical division. Lineage restriction is demonstrated within developing clones which are sensitive to the local environment. The invention also includes such cells which are transfected with foreign nucleic acid, e.g., to produce an immortalized neural stem cell, and immortalized cell lines which are capable of subsequent disimmortalization. The invention further includes transplantation assays which allow for the identification of mammalian multipotent neural stem cells from various tissues and methods for transplanting mammalian neural stem cells and/or neural or glial progenitors into mammals. A novel method for detecting antibodies to neural cell surface markers is disclosed as well as a monoclonal antibody to mouse LNGFR.
US Patent Website:Click Here for Full Text of Patent
Title Number:US5849553
Application Number:US1995000485612
Date Filed:07/06/1995
Date Published:15/12/1998
Assignee:California Institute of Technology, Pasadena, CA, USA


 
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