Methods comprising apoptosis inhibitors for the generation of transgenic pigs

Stem Cell Clinic
Patient Application
FAQ
Contact
Locations
News
Videos
Research
Patents
3D Culture/Scaffold
Administration
Differentiation
Expansion
Extraction/Preservation
Mobilization
Type
USE
 


Stem Cell Related Patent Number US5858740

Title:Method for production of high titer virus and high efficiency retroviral mediated transduction of mammalian cells
Inventors:Finer, Mitchell H.; San Carlos, CA, USA
Roberts, Margo R.; San Francisco, CA, USA
Dull, Thomas J.; San Francisco, CA, USA
Zsebo, Krisztina M.; Woodside, CA, USA
Qin, Lu; Foster City, CA, USA
Farson, Deborah A.; Oakland, CA, USA
Summary:Described herein are replication-defective retroviral vectors, methods and uses thereof. The invention relates to a novel retroviral packaging system in which retroviral packaging constructs and packagable vector transcripts are produced from high expression plasmids by transfection in human cells from which high titers of recombinant retrovirus are generated. Further disclosed are methods by which the novel retroviral constructs may be utilized to transduce primary human cells, including T cells and human hematopoietic stem cells, with foreign genes by cocultivation. Therapeutic applications are provided for the rapid production of high viral supernatants, for the expression of foreign genes via the efficient transduction of mammalian target cells that are refractory to transduction by conventional means, and for use in gene therapy.
Abstract:The invention provides a novel retroviral packaging system, in which retroviral packaging constructs and packagable vector transcripts are produced from high expression plasmids by transfection in human cells. High titers of recombinant retrovirus are produced in infected cells. The methods of the invention include the use of the novel retroviral constructs to transduce primary human cells, including T cells and human hematopoietic stem cells, with foreign genes by cocultivation at high efficiencies. The invention is useful for the rapid production of high viral supernatants, and to transduce with high efficiency cells that are refractory to transduction by conventional means.
US Patent Website:Click Here for Full Text of Patent
Title Number:US5858740
Application Number:US1995000438582
Date Filed:10/05/1995
Date Published:12/01/1999
Assignee:Cell Genesys, Inc., Foster City, CA, USA


 
Copyright © 2007 The Institute for Cellular Medicine  8/5/2020