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| Title: | Method for production of high titer virus and high efficiency retroviral mediated transduction of mammalian cells |
| Inventors: | Finer, Mitchell H.; San Carlos, CA, USA Roberts, Margo R.; San Francisco, CA, USA Dull, Thomas J.; San Francisco, CA, USA Zsebo, Krisztina M.; Woodside, CA, USA Qin, Lu; Foster City, CA, USA Farson, Deborah A.; Oakland, CA, USA |
| Summary: | Described herein are replication-defective retroviral vectors, methods and uses thereof. The invention relates to a novel retroviral packaging system in which retroviral packaging constructs and packagable vector transcripts are produced from high expression plasmids by transfection in human cells from which high titers of recombinant retrovirus are generated. Further disclosed are methods by which the novel retroviral constructs may be utilized to transduce primary human cells, including T cells and human hematopoietic stem cells, with foreign genes by cocultivation. Therapeutic applications are provided for the rapid production of high viral supernatants, for the expression of foreign genes via the efficient transduction of mammalian target cells that are refractory to transduction by conventional means, and for use in gene therapy. |
| Abstract: | The invention provides a novel retroviral packaging system, in which retroviral packaging constructs and packagable vector transcripts are produced from high expression plasmids by transfection in human cells. High titers of recombinant retrovirus are produced in infected cells. The methods of the invention include the use of the novel retroviral constructs to transduce primary human cells, including T cells and human hematopoietic stem cells, with foreign genes by cocultivation at high efficiencies. The invention is useful for the rapid production of high viral supernatants, and to transduce with high efficiency cells that are refractory to transduction by conventional means. |
| US Patent Website: | Click Here for Full Text of Patent |
| Title Number: | US5858740 |
| Application Number: | US1995000438582 |
| Date Filed: | 10/05/1995 |
| Date Published: | 12/01/1999 |
| Assignee: | Cell Genesys, Inc., Foster City, CA, USA |