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Stem Cell Related Patent Number US5861171

Title:Adeno-associated viral (AAV) liposomes and methods related thereto
Inventors:Philip, Ramila; Redwood City, CA, USA
Lebkowski, Jane; Portola Valley, CA, USA
Summary:Described herein is a novel composition and methods of its use in genetic manipulation. The invention relates to a T cell transfected by a composition comprised of a cationic liposome and a double-stranded circular plasmid DNA vector lacking a selectable marker and encoding at least one inverted terminal repeat from an adeno-associated virus (AAV), a promoter other than an AAV promoter, and a genetic sequence of interest. Further disclosed are methods of genetic manipulation by which genetic material may be introduced into stem cells, T cells, primary tumor cells and tumor cell lines by use of the AAV liposomes. Therapeutic applications are included for the treatment of cancer, HIV infection, autoimmune disease and genetic abnormalities. This invention represents a continuation of other applications filed by the same assignee and inventors.
Abstract:A composition for genetic manipulation which comprises a liposome comprised of lipid material, and adeno-associated viral (AAV) material. Typically, the AAV material is plasmid, and comprises a terminal repeat of the AAV genome. Methods are disclosed for introducing genetic material into cells by use of AAV liposomes. Accordingly, genetic material was introduced and integrated into stem cells, T cells, primary tumor cells, or tumor cell lines.
US Patent Website:Click Here for Full Text of Patent
Title Number:US5861171
Application Number:US1995000458342
Date Filed:02/06/1995
Date Published:19/01/1999
Assignee:Rhone-Poulenc Rorer Pharmaceuticals Inc., Collegeville, PA, USA

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