Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US5866115

Title:Process for preparing dendritic cells, cells thus produced and containers for carrying out this process
Inventors:Kanz, Lothar; Freiburg, Germany
Brugger, Wolfram; Kirchzarten, Germany
Henschler, Reinhard; Freiburg, Germany
Kohler, Gabriele; Freiburg, Germany
Schaefer, Hans-Eckart; Freiburg, Germany
Lindemann, Albrecht; Solden, Germany
Mertelsmann, Roland; Freiburg, Germany
Mackensen, Andreas; Freiburg, Germany
Fisch, Paul; Freiburg, Germany
Herbst, Birgit; Schallstadt, Germany
Summary:Described herein is a process for producing dendritic cells from peripheral blood precursor cells. The invention relates to methods by which such precursor cells are isolated and the CD34 antigen-expressing cells contained therein are enriched and expanded ex vivo in the presence of cytokines and hematopoietic growth factors such as erythropoietin, various interleukins and a granulocyte macrophage colony stimulating factor. Further disclosed are properties of antigen-presenting dendritic cells which arise after 10 to 20 days from the expanded precursor cells, and which may be purified further if necessary. Therapeutic applications in immunotherapy are included.
Abstract:Dendritic cells are of interest therapeutically as antigen-presenting cells. A process is disclosed in which peripheral blood cells are first isolated and the CD 34 antigen-expressing blood progenitor cells which they contain are then enriched. These enriched cells are expanded ex-vivo using a combination of haematopoietic growth factors and cytokines. Over a period of 10-20 days, they give rise, in particular to dendritic cells which, where appropriate, can be purified still further. These cells are functionally active with regard to the ability to present antigen.
US Patent Website:Click Here for Full Text of Patent
Title Number:US5866115
Application Number:US1996000727495
Date Filed:11/10/1996
Date Published:02/02/1999
Assignee:Klinikum Der Albert-Ludwigs-Universitat Freiburg, Freiburg, Germany


 
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