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| Title: | Process for preparing dendritic cells, cells thus produced and containers for carrying out this process |
| Inventors: | Kanz, Lothar; Freiburg, Germany Brugger, Wolfram; Kirchzarten, Germany Henschler, Reinhard; Freiburg, Germany Kohler, Gabriele; Freiburg, Germany Schaefer, Hans-Eckart; Freiburg, Germany Lindemann, Albrecht; Solden, Germany Mertelsmann, Roland; Freiburg, Germany Mackensen, Andreas; Freiburg, Germany Fisch, Paul; Freiburg, Germany Herbst, Birgit; Schallstadt, Germany |
| Summary: | Described herein is a process for producing dendritic cells from peripheral blood precursor cells. The invention relates to methods by which such precursor cells are isolated and the CD34 antigen-expressing cells contained therein are enriched and expanded ex vivo in the presence of cytokines and hematopoietic growth factors such as erythropoietin, various interleukins and a granulocyte macrophage colony stimulating factor. Further disclosed are properties of antigen-presenting dendritic cells which arise after 10 to 20 days from the expanded precursor cells, and which may be purified further if necessary. Therapeutic applications in immunotherapy are included. |
| Abstract: | Dendritic cells are of interest therapeutically as antigen-presenting cells. A process is disclosed in which peripheral blood cells are first isolated and the CD 34 antigen-expressing blood progenitor cells which they contain are then enriched. These enriched cells are expanded ex-vivo using a combination of haematopoietic growth factors and cytokines. Over a period of 10-20 days, they give rise, in particular to dendritic cells which, where appropriate, can be purified still further. These cells are functionally active with regard to the ability to present antigen. |
| US Patent Website: | Click Here for Full Text of Patent |
| Title Number: | US5866115 |
| Application Number: | US1996000727495 |
| Date Filed: | 11/10/1996 |
| Date Published: | 02/02/1999 |
| Assignee: | Klinikum Der Albert-Ludwigs-Universitat Freiburg, Freiburg, Germany |