Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US5876956

Title:Methods for identification or purification of cells containing an enzymatic intracellular marker
Inventors:Jones, Richard J.; Bel Air, MD, USA
Colvin, O. Michael; Baltimore, MD, USA
Hilton, John; Parkton, MD, USA
Sharkis, Saul; Towson, MD, USA
Summary:Described herein is an enriched cellular composition comprised of human pluripotent hematopoietic stem cells (HSCs) and an enzymatic cellular marker. The invention relates to methods by which an enriched stem cell composition is produced according to a nontoxic process in which the cell mixture is contacted with a cell-permeable, fluorescent nonpolar compound that reacts upon contact with the intracellular enzymes aldehyde dehydrogenase or phosphokinase so as to form a biocompatible fluorescent product that is polar and non-permeable to the membrane of the stem cells. Further disclosed are methods of isolating the cells containing the marker via fluorescent cell sorting. Therapeutic applications are included in bone marrow transplantation and in the treatment of immune deficiency disorders.
Abstract:Production of a stem cell enriched cell composition using essentially non-toxic methodology comprising contacting a cell mixture with a cell-permeable, fluorescent non-polar compound that reacts upon contact with intracellular enzyme aldehyde dehydrogenase or phosphokinase found substantially exclusively in stem cells so as to form a biocompatible fluorescent product that is polar and non-permeable to the membrane of the stem cells, and isolating the cells containing the marker by fluorescent cell sorting.
US Patent Website:Click Here for Full Text of Patent
Title Number:US5876956
Application Number:US1995000440656
Date Filed:15/05/1995
Date Published:02/03/1999
Assignee:Johns Hopkins University School of Medicine, Baltimore, MD, USA


 
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